Dissertation/Thesis Abstract

Identification and characterization of ets family gene members in Ophiocoma wendtii
by Hamilton, Melissa Kaye, M.S., California State University, Long Beach, 2013, 91; 1523052
Abstract (Summary)

Adult echinoderms form a mineralized skeleton, but only sea urchins and brittle stars form larval skeletons. In the sea urchin Strongylocentrotus purpuratus, the gene regulatory network (GRN) leading to skeleton formation has been characterized. This S. purpuratus GRN includes several members of the ets family, including Erg, Ets1/2 and Gabp. The brittle star Ophiocoma wendtii forms an embryonic skeleton similar to S. purpuratus. The goal of this proposal is to see if expression of the ets family members is conserved as part of the skeletogenic GRN in O. wendtii. Four genes were identified in O. wendtii; homologous to S. purpuratus Erg, Ets1/2, Ets4 and Gabp based on phylogenetic analysis. The coding sequences of these O. wendtii genes were obtained and their temporal expression was determined. These results suggest that sea urchins and brittle stars share a GRN leading to skeleton formation that has been activated in the embryos of both.

Indexing (document details)
Advisor: Livingston, Brian
Commitee: Eldon, Elizabeth, Malcomber, Simon
School: California State University, Long Beach
Department: Biological Sciences
School Location: United States -- California
Source: MAI 52/01M(E), Masters Abstracts International
Subjects: Molecular biology, Evolution and Development
Keywords: Brittle stars, ETS gene family, Ophiocoma wendtii, Sea urchins, Strongylocentrotus purpuratus, Transcription factors
Publication Number: 1523052
ISBN: 978-1-303-20225-4
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