Through the use of a novel immunodetection assay, our lab identified a series of mutants that internally accumulate the precursor form of the vacuolar hydrolase carboxypeptidase Y (CPY), a phenotype that suggests a defect at the late endosome to vacuole ( ENV) interface of the biosynthetic pathway. This study focuses on two of the novel genes identified: ENV10 and ENV11 and is the first to establish the cellular localization of Env10p in the endoplasmic reticulum. Assays of vacuole and lipid droplet morphology, as well as growth characterization under various stressors demonstrate Env10p has a role in vacuolar protein trafficking and endomembrane system integrity and may operate in a parallel or compensatory manner to Env9p. This study also confirms that Env11p localizes to the nucleus in a saturable fashion where it may be involved in transcriptional regulation of genes involved in vacuole events in conjunction with Vid22p and Tbf1p.
|Commitee:||Khatra, Balwant S., Lee-Fruman, Kay|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 52/01M(E), Masters Abstracts International|
|Subjects:||Molecular biology, Cellular biology|
|Keywords:||ENV10, ENV11, ENV9, Lysosomal trafficking, Saccharomyces cerevisiae, Vacuole|
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