Little is known about the genomic differences between clinical and environmental isolates of Burkholderia pseudomallei, the causative agent of melioidosis. With the advent of whole genome sequencing, the ability to sequence numerous genomes fairly inexpensively allows a thorough analysis of many genomes to be undertaken. In this study we sequenced 100 (50 clinical and 50 environmental) B. pseudomallei isolates from Thailand using Illumina’s next generation sequencing technique. While previous studies have attempted to determine differences between clinical and environmental isolates the use of a clinical isolate alone to determine probable genes could greatly limit the search. The pangenome used to produce the probes for in silico analysis in this study uses globally diverse clinical and environmental samples. Looking at the presence or absence of 2,651 genes across these genomes did not uncover any genes that could play a role in distinguishing clinical isolates from environmental. An NMDS plot based on the genes that could be potentially useful in differentiating the Thai samples illustrated groupings of clinical and environmental isolates, however, there was still a large section of overlap. When these isolates were put in a global context and an NMDS plot was produced using the larger set of differentiating genes a similar pattern was seen.
|Advisor:||Tuanyok, Apichai, Keim, Paul|
|Commitee:||Keim, Paul, Wagner, David|
|School:||Northern Arizona University|
|School Location:||United States -- Arizona|
|Source:||MAI 51/06M(E), Masters Abstracts International|
|Keywords:||Burkholderia pseudomallei, Clinical, Environmental, Genomic, Thailand|
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