DNA stable isotope probing directly identified microbial utilizers of 13C-labeled lignocellulose, a major component of Spartina foliosa. Overall, 146 species were detected, of which only 12 were shared by both enriched and non-enriched communities. Functionally relevant bacteria from the 13C-labeled community include representatives such as Desulfosarcina, Spirochaeta, and Kangiella. Enriched experiments determined large contributions of microalgal-derived carbon and small contributions of Spartina foliosa-derived carbon to the diets of sediment invertebrates. Invertebrate exclusion experiments showed that organisms without direct access had a slightly elevated δ 15N signature, suggesting small amounts of decomposed S. foliosa-derived carbon are liberated via microbial mediation. This is the first study of our knowledge to use DNA stable isotope probing in salt marsh sediments and with enriched lignocellulose to uncover microbial utilizers of macrophyte derived carbon and make a potential link between source and consumer to describe a more complete benthic salt marsh food web that includes microbes.
|Advisor:||Dillon, Jesse. G.|
|Commitee:||Allen, Bengt, Whitcraft, Christine R.|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 51/05M(E), Masters Abstracts International|
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