The redistribution of nitrogen from old leaves to young leaves during senescence is crucial for plant survival. The Arabidopsis thaliana chloroplast residential protease S33Ab (At5g11650) was predicted to contribute to the degradation of chloroplast stromal proteins during senescence by bioinformatics tools. Rubisco degradation did not co-segregate with the T-DNA insertion mutant s33Ab. The location of T-DNA insertions into S33Ac (At1g18360) and S33Ad (At1g73480) were confirmed by sequencing. Total leaf proteins from Ab, Ac, Ad single, Ab/Ac, Ab/Ad, Ac/Ad double and Ab/Ac/Ad triple mutants were isolated from senescing leaves, subjected to immunoblot analysis to quantify Rubisco large subunit (LSU), glutamine synthase 2 (GS2), Rubisco activase (RCA), and light harvesting complex protein for photosystem II, b (Lhcb1) and compared to wild type. There is no significant difference among all the genotypes. This genetic analysis shows that S33Ab and its two closely related homologs, S33Ac and S33Ad do not individually contribute or work additively in chloroplast stromal protein degradation.
|Commitee:||Eldon, Elizabeth, Malcomber, Simon|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 51/05M(E), Masters Abstracts International|
|Subjects:||Molecular biology, Plant biology, Genetics|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be