Dissertation/Thesis Abstract

Genetic analysis to determine if S33Ab, S33Ac and S33Ad chloroplast proteases contribute to stromal protein degradation during Arabidopsis thaliana leaf senescence
by Dou, Xiaoyu, M.S., California State University, Long Beach, 2013, 72; 1522627
Abstract (Summary)

The redistribution of nitrogen from old leaves to young leaves during senescence is crucial for plant survival. The Arabidopsis thaliana chloroplast residential protease S33Ab (At5g11650) was predicted to contribute to the degradation of chloroplast stromal proteins during senescence by bioinformatics tools. Rubisco degradation did not co-segregate with the T-DNA insertion mutant s33Ab. The location of T-DNA insertions into S33Ac (At1g18360) and S33Ad (At1g73480) were confirmed by sequencing. Total leaf proteins from Ab, Ac, Ad single, Ab/Ac, Ab/Ad, Ac/Ad double and Ab/Ac/Ad triple mutants were isolated from senescing leaves, subjected to immunoblot analysis to quantify Rubisco large subunit (LSU), glutamine synthase 2 (GS2), Rubisco activase (RCA), and light harvesting complex protein for photosystem II, b (Lhcb1) and compared to wild type. There is no significant difference among all the genotypes. This genetic analysis shows that S33Ab and its two closely related homologs, S33Ac and S33Ad do not individually contribute or work additively in chloroplast stromal protein degradation.

Indexing (document details)
Advisor: Brusslan, Judy
Commitee: Eldon, Elizabeth, Malcomber, Simon
School: California State University, Long Beach
Department: Biological Sciences
School Location: United States -- California
Source: MAI 51/05M(E), Masters Abstracts International
Subjects: Molecular biology, Plant biology, Genetics
Publication Number: 1522627
ISBN: 978-1-303-02039-1
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