Mycofiltration has been proposed as an ecologically sensitive and economically practical solution for removing nutrients from waters. However, fungal systems are known to contribute to nitrous oxide (N₂O) emissions. N ₂O is currently the dominant ozone depletor and a powerful green house gas. Five fungal species were propagated under various wastewater concentrations and their emissions were sampled three times after inoculation. Only Pleurotus ostreatus consistently showed higher N₂O concentrations compared to controls but only during the third sampling. Only Coprinus comatus during the second sampling showed a significant correlation between TAN and N₂O. C. comatus may emit N ₂O with increased nitrogen availability. This research indicates P. ostreatus and C. comatus may be poor candidates for mycofiltration due to N₂O emission potential, though the impact has yet to be determined. Stropharia rugosoannulata, Hypsizygus ulmarius and Trametes versicolor are recommended for future mycofiltration research, especially T. versicolor as it may consume N₂O.