Dissertation/Thesis Abstract

Photonic and Ablation-based Ionization for the Analysis of Single Cells and Organelles
by Stolee, Jessica A., Ph.D., The George Washington University, 2013, 193; 3549849
Abstract (Summary)

A systemic understanding of the dynamic cellular processes in living organisms requires highly sensitive techniques that can rapidly detect and identify diverse biomolecules. Owing to the specificity and sensitivity of mass spectrometric analysis, it has become an indispensable tool for chemical analysis. The metabolic analysis of single cells and subcellular organelles is an emerging field that aims at studying cellular heterogeneity and its effect on the response of an organism to disease and environmental influences. Technical advances in mass spectrometry are required to make significant strides in this field. This dissertation describes my achievements based on photonic nanostructures and mid-infrared laser ablation for the mass spectral analysis of single cells and organelles.

Indexing (document details)
Advisor: Vertes, Akos
Commitee: Dowd, Cynthia, Licht, Stuart, Miller, John H., Yergey, Alfred
School: The George Washington University
Department: Chemistry
School Location: United States -- District of Columbia
Source: DAI-B 74/05(E), Dissertation Abstracts International
Source Type: DISSERTATION
Subjects: Analytical chemistry, Physical chemistry
Keywords: Electrospray ionization, Mass spectrometry, Matrix-free ionization, Nanophotonics, Organelles, Single cells
Publication Number: 3549849
ISBN: 978-1-267-86571-7
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