Dissertation/Thesis Abstract

Cloning of the gene for metallothionein from Artemia and challenges with cloning the promoter
by Jordaan, Gwen R., M.Sc., California State University, Long Beach, 2012, 93; 1521626
Abstract (Summary)

Four metallothionein (MT) isoforms have previously been identified in the brine shrimp Artemia. The goals of this study were to isolate at least one of the MT genes, clone a MT promoter region, and characterize the cis-acting sequences that regulate MT gene expression. PCR of Artemia genomic DNA amplified a product containing a MT gene with no internal introns. Three different approaches were made to clone the MT promoter. Firstly, conventional cloning of size-selected genomic DNA into a dephosphorylated vector produced empty clones or no clones. The use of a novel linear cloning vector produced many recombinant clones, but screening of the DNA library did not identify a MT promoter sequence. Lastly, genome walking by PCR amplified a sequence that lacked a TAT A box and metal response elements. A blast search identified the sequence as a mitochondrial hydroxyacylglutathione hydrolase found in marine organisms.

Indexing (document details)
Advisor: Acey, Roger A.
Commitee: Acey, Roger A., McAbee, Douglas D., Weers, Paulus M.
School: California State University, Long Beach
Department: Chemistry and Biochemistry
School Location: United States -- California
Source: MAI 51/04M(E), Masters Abstracts International
Subjects: Molecular biology, Biochemistry
Publication Number: 1521626
ISBN: 978-1-267-79055-2
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