Little is known about the global prevalence of halophiles in coastal waters. In this study, I hypothesized there would be changes in the abundance and community structure of halophiles isolated from seawater samples along the Pacific coast of North America due to seasonal and geographic variation in conditions. Twelve sandy beaches were sampled over 2,600 km in winter and summer and monthly samples were taken at Huntington Beach, CA. Heterotrophic halophiles were isolated using hypersaline media and identified using 16S rRNA sequencing. For comparison, isolates were obtained using artificial seawater medium. Isolates belonged to four bacterial phyla, Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. Seasonal differences in abundance and community structure were detected. Isolates from artificial seawater medium had narrow salinity tolerance ranges, while those isolated from hypersaline media were euryhaline. This study supports the widespread occurrence of cultivable halotolerant bacteria in coastal settings displaying seasonal patterns in their community structure.
|Commitee:||Allen, Bengt, Pernet, Bruno|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 51/04M(E), Masters Abstracts International|
|Subjects:||Microbiology, Biological oceanography|
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