Dissertation/Thesis Abstract

Seasonal diversity and salinity tolerance of cultivable heterotrophic marine bacteria along the Pacific coast of North America
by Schaadt, Erin Celeste, M.S., California State University, Long Beach, 2012, 63; 1521639
Abstract (Summary)

Little is known about the global prevalence of halophiles in coastal waters. In this study, I hypothesized there would be changes in the abundance and community structure of halophiles isolated from seawater samples along the Pacific coast of North America due to seasonal and geographic variation in conditions. Twelve sandy beaches were sampled over 2,600 km in winter and summer and monthly samples were taken at Huntington Beach, CA. Heterotrophic halophiles were isolated using hypersaline media and identified using 16S rRNA sequencing. For comparison, isolates were obtained using artificial seawater medium. Isolates belonged to four bacterial phyla, Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. Seasonal differences in abundance and community structure were detected. Isolates from artificial seawater medium had narrow salinity tolerance ranges, while those isolated from hypersaline media were euryhaline. This study supports the widespread occurrence of cultivable halotolerant bacteria in coastal settings displaying seasonal patterns in their community structure.

Indexing (document details)
Advisor: Dillon, Jesse
Commitee: Allen, Bengt, Pernet, Bruno
School: California State University, Long Beach
Department: Biological Sciences
School Location: United States -- California
Source: MAI 51/04M(E), Masters Abstracts International
Subjects: Microbiology, Biological oceanography
Publication Number: 1521639
ISBN: 978-1-267-79068-2
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