The goal of my thesis work was to create theranostic nanoparticles for tumor imaging and therapy based on biodegradable polyacrylamide (PAA) nanoparticles. In order to achieve this goal; my thesis is divided into six parts. The first chapter is an overview of the current research in the area of theranostics, synthetic and nanotechnology based. The second chapter details the various loading methodologies present, their loading efficiency, and photophysical characterization. The post-loading method showed the highest loading efficiency. The third chapter investigates the in vitro and in vivo Phototherapeutic efficacy of these nanoparticles. Chapters 2 and 3 showed that the post-loading methodology produced the highest phototherapeutic activity. The fourth chapter utilizes biodegradable polyacrylamide nanoparticle for the formation of a theranostic nanoconstruct for near-infrared fluorescence imaging and photodynamic therapy (PDT). By varying the ratio of photosensitizer to cyanine dye a single nanoconstruct was afforded for near-infrared fluorescence and PDT. The sixth and final chapter highlights the use of PAA nanoparticles for PET/near-infrared fluorescence with an option of PDT. The post-loading methodology showed a remarkable enhancement in the uptake of the radiolabeled PS to the tumor while minimizing the PS accumulation in residual organs. The fifth chapter describes the utility of porphyrin based PS and PAA nanoparticles for photoacoustic imaging. A conjugation of the porphyrin based PS to the surface of the PAA nanoparticles caused the enhanced aggregation of the PS, which was found to result in the increased photoacoustic signal. Overall, it was shown that polyacrylamide nanoparticles provided a platform to reach our goal.
|Advisor:||Pandey, Ravindra K.|
|Commitee:||Ohulchanskyy, Tymish, Sunar, Ulas, Yao, Rutao|
|School:||State University of New York at Buffalo|
|School Location:||United States -- New York|
|Source:||DAI-B 74/02(E), Dissertation Abstracts International|
|Subjects:||Biomedical engineering, Medical imaging, Nanotechnology|
|Keywords:||Fluorescence imaging, PET imaging, Photoacoustic tomography, Photodynamic therapy, Polyacrylamide|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be