Gold nanoparticles are considered to have great potential in drug delivery and photothermal therapy applications due to their chemical stability and biocompatibility. For these applications, highly ordered structures are desired. Here we show a novel method of organizing gold nanoparticles with rigid polymers such as collagen mimetic peptides into ordered assemblies. Collagen mimetic peptides have amino acid sequences that mimic collagen, but allow for greater experimental control due to their smaller size and reversible helix-to-coil transition. In this work, 2.0 ± 0.3 nm gold nanoparticles with surface functionalized carboxylic acid groups (AuTP) and 5.4 ± 0.6 nm gold nanoparticles with surface functionalized amine groups (AuCYS) were synthesized and coupled to collagen mimetic peptides via N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) coupling agent. A complimentary peptide was used to form a collagen mimetic peptide in a triple helix conformation, therefore, directly linking AuTP and AuCYS. Transmission electron microscopy (TEM) showed the distance between AuTP and AuCYS to be 7. 7 ± 1.5 nm.
|Commitee:||Shon, Y oung-Seok, Weers, Paul|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 51/03M(E), Masters Abstracts International|
|Subjects:||Biochemistry, Inorganic chemistry|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be