The chemokine-like factor super family (CKLFSF) represents a new class of signaling peptide that is involved in macrophage activation and development. A novel down-regulated gene found in rat macrophages is highly homologous to the third family class and is designated rat CKLFSF3. Translation of the largest open reading frame predicts a 24-kDa peptide designated rCKLFSF3 peptide. Isolated murine macrophage nuclei subjected to a 0.3 M salt gradient results in the extraction of a 24 kDa nuclear protein as detected using anti-rCKLFSF3 antibodies. The level of rCKLFSF3 peptide is also shown to become undetectable hours after macrophage activation. The amino acid sequence predicts a membrane-bound peptide but possessing the DNA binding leucine zipper motif and transcription regulating LxxLL sequence. This suggests that rCKLFSF3 peptide exists as a nuclear membrane bound transcription factor that is down-regulated after activation.
|Advisor:||Acey, Roger A.|
|Commitee:||Jadus, Martin, McAbee, Douglas|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 51/03M(E), Masters Abstracts International|
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