Dissertation/Thesis Abstract

Comparative virulence of enterotoxigenic non-Escherichia coli Enterobacteriaceae species from patients with travelers' diarrhea
by Raines, Kirsten M., Ph.D., The University of Texas School of Public Health, 2012, 75; 3521849
Abstract (Summary)

Background: Family members of Enterobacteriaceae are found in small numbers associated with acute diarrhea. These species are sometimes mistaken for ETEC.

Methods: Forty-four non-E. coli species from travelers' diarrhea are compared to 30 strains of Escherichia coli (ETEC) and 30 strains of normal flora E. coli. Tissue culture supernatants were assayed by enzyme-linked immunosorbent assay for amounts of IL-8, IL-1, and IL-1ra. Amounts of heat-stable (ST) and heat-labile (LT) enterotoxins were assayed from cell culture supernatants by enzyme-linked immunoassay. PCR was use to determine which species was positive colonization factor antigens, CFA/I, CS3, and CS6.

Results: Normal flora E. coli significantly induced the production of more IL-8 than non- E. coli and ETEC. Normal E. coli also induced the production of more IL-1and IL-1ra than ETEC. Non-E. coli produced more ST than ETEC. A small percentage of enterotoxigenic non- E. coli gram negatives and ETEC were positive for CFA/I and CS6. None of the strains were positive for CS3.

Conclusions: Non-E. coli enterotoxigenic gram negatives were similar to ETEC in their virulence factors. Identification and further study of these non-E.coli strains is important for understanding their pathogenic role in acute diarrhea.

Indexing (document details)
Advisor: DuPont, Herbert L.
Commitee: Brown, Eric, Jiang, Zhi-Dong, Rodin, Andrei
School: The University of Texas School of Public Health
Department: Epidemiology & Disease Control
School Location: United States -- Texas
Source: DAI-B 74/01(E), Dissertation Abstracts International
Subjects: Microbiology
Keywords: Colonization factor antigens, Cytokines, Diarrhea, Enterobacteriaceae, Enterotoxins
Publication Number: 3521849
ISBN: 978-1-267-53338-8
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