Among the main mechanisms of evolution we find adaptation, genetic drift, gene flow, mutation, natural selection and speciation. The following thesis explores bacterial mutation rate, mutants, mutators, and how competition acts as a driving force of adaptation.
In chapter 1, the system used in reference for mutation is the development of Pseudomonas aeruginosa antibacterial resistance in the Cystic Fibrosis patients. Current methods for determining mutation rate are quite involved mathematically or complicated experimentally. A new method for estimating mutation rate is discussed and compared with two other widely used methods.
In chapter 2, a microarray data set of different strains are compared. We find that the data set has strains that have sections of deleted genes. The two categories are analyzed across two different tests and genetic deletions are compared. The strains are also known to be mutators or non-mutators and the data will be used for strain classification.
In chapter 3, a competition model of killer and non-killer yeast is used to predict the outcome of competition in a continuous culture under nutrient limitation (e.g. a chemostat). This research explores possible detrimental effect on fitness when yeast harbor the killer virus under chemostat conditions and when a non-killer yeast strain can out compete a killer-strain of yeast.
|Commitee:||Bardsley, John, Miller, Scott, Rosenzweig, Frank, Stone, Emily|
|School:||University of Montana|
|School Location:||United States -- Montana|
|Source:||DAI-B 73/12(E), Dissertation Abstracts International|
|Keywords:||Bacterial mutations, Chemostat, Competition, Microarrays, Mutation rates|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be