The objective of this thesis was to identify genes involved in protein transport at the late phase of the vacuolar protein trafficking pathway, specifically the late endosome to lysosome/vacuole, in Saccharomyces cerevisiae. To broaden the number of genes identified at this late phase of the vacuolar protein trafficking pathway, a genome-wide screen using the yeast MATα deletion strain collection of non-essential genes was undertaken. The deletion collection was subjected to two sets of immunodetection screens (primary and secondary screens). Initially, 4,760 deletion mutants were subjected to the primary screen, and results revealed 338 mutants that displayed defects in the processing of the vacuolar protease carboxypeptidase Y (CPY). Subsequently, these mutants were subjected to a secondary screen which identified mutants that internally accumulate proCPY and were named env mutants. This study uncovered 12 ENV genes which include one major group of uncharacterized genes, one group of genes involved in mitochondrial events, and four genes involved in cellular processes such as cell cycle, osmoregulation, and translation. In addition to env mutants, we also identified one new vacuolar protein sorting mutant (vps mutant).
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 51/01M(E), Masters Abstracts International|
|Subjects:||Molecular biology, Cellular biology|
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