Dissertation/Thesis Abstract

A comprehensive study of mammalian SNAG transcription factor family members
by Chiang, Cindy, Ph.D., Florida Atlantic University, 2012, 198; 3520016
Abstract (Summary)

Transcriptional regulation by the family of SNAG (Snail/Gfi-1) zinc fingers has been shown to play a role in various developmental states and diseases. These transcriptional repressors have function in both DNA- and protein-binding, allowing for multiple interactions by a single family member. This work aims to characterize the SNAG members Slug, Smuc, Snail, Scratch, Gfi-1, Gfi-1B, and IA-1 in terms of both DNA-protein and protein-protein interactions.

The specific DNA sequences to which the zinc finger regions bind were determined for each member, and a general consensus of TGCACCTGTCCGA, was developed for four of the members. Via these studies, we also reveal thebinding affinities of E-box (CANNTG) sequences to the members, since this core is found for multiple members’ binding sites.

Additionally, protein-protein interactions of SNAG members to other biological molecules were investigated. The Slug domain and Scratch domain have unknown function, yet through yeast two-hybrid screening, we were able to determine protein interaction partners for them as well as for other full length SNAG members. These protein-interacting partners have suggested function as corepressors during transcriptional repression.

The comprehensive information determined from these studies allow for a better understanding of the functional relationship between SNAG-ZFPs and other genes. The collected data not only creates a new profile for each member investigated, but it also allows for further studies to be initiated from the results.

Indexing (document details)
Advisor: Ayyanathan, Kasirajan
Commitee:
School: Florida Atlantic University
School Location: United States -- Florida
Source: DAI-B 73/11(E), Dissertation Abstracts International
Source Type: DISSERTATION
Subjects: Molecular biology
Keywords: DNA binding sites, Protein-protein interactions, SNAG domain, Transcriptional regulation
Publication Number: 3520016
ISBN: 9781267475022
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