Lipofuscin concentration analysis has been supported as a useful way to estimate age in crustacean populations and has been shown to be more reliable than traditional age determination by body size. This study examined the lipofuscin concentration from the brains of twenty-five, 2-year-old American lobsters held in situ at six sites off the coast of Maine, representing different temperature regimes. Lobsters were collected and preserved in 10% neutral buffered formalin. After dissection, brain tissues were prepared through a series of dehydrating ethanol baths and cleared with xylene. The brain tissue was embedded in paraffin wax, sectioned and slide mounted for analysis using a confocal microscope and image analysis software (Image J™). The lipofuscin concentrations did not differ significantly between the site locations suggesting lipofuscin concentration is not temperature dependent.
|Advisor:||Grace, Sean P.|
|School:||Southern Connecticut State University|
|School Location:||United States -- Connecticut|
|Source:||MAI 50/05M, Masters Abstracts International|
|Subjects:||Ecology, Aquatic sciences|
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